DLG1 anticorps (N-Term)

N° du produit ABIN6658116

Détail du produit anti-DLG1 anticorps

Antigène: DLG1 (Discs, Large Homolog 1 (DLG1))

Épitope: N-Term

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DLG1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Anti-SAP97 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SAP97 from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. No cross-reactivity against PSD95, SAP102 and Chapsyn110 expressed in transfected cells. Cross-reactivity with SAP97 from other sources has not been determined. Ion Channels research.

Immunogène:

Immunogen: SAP97 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an n-terminal region of rat SAP97.

Immunogen Type: Recombinant Protein

Clone: S64-15

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-SAP97 Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~130 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur DLG1

Antigène: DLG1 (Discs, Large Homolog 1 (DLG1))

Autre désignation: SAP97 (DLG1 Produits)

Synonymes: anticorps DLG1, anticorps Dlgh1, anticorps SAP97, anticorps B130052P05Rik, anticorps E-dlg/SAP97, anticorps SAP-97, anticorps mKIAA4187, anticorps DLGH1, anticorps dJ1061C18.1.1, anticorps hdlg, anticorps discs large MAGUK scaffold protein 1, anticorps discs, large homolog 1 (Drosophila), anticorps DLG1, anticorps Dlg1

Sujet:

Synonyms: Dlgh1, SAP97, Dlg1, Synapse-associated protein 97, Disks large homolog 1

Background: Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif (2). Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Gene Name: Dlg1

ID gène: 25252

NCBI Accession: NP_036920

UniProt: Q62696

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response