rRNA 2'-O-Methyltransferase Fibrillarin (FBL) anticorps

N° du produit ABIN6658299

N° du produit (Fournisseur): 900-301-d39

Cet anticorps Souris Monoclonal détecte spécifiquement dans IHC, WB et FM. Il présente une réactivité envers Saccharomyces cerevisiae.

Aperçu rapide pour rRNA 2'-O-Methyltransferase Fibrillarin (FBL) anticorps (ABIN6658299)

Antigène: rRNA 2'-O-Methyltransferase Fibrillarin (FBL)

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM)

Clone: 38F3

Détail du produit

N° du produit (Fournisseur): 900-301-d39

Fournisseur: Rockland

Fonction: Fibrillarin Antibody

Réactivité croisée (Details): Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate.

Purification: Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein.

Immunogène: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

Isotype: IgG1

Information d'application

Indications d'application:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Fibrillarin (Mouse) Antibody is tested for use in Western Blotting, ICC and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: rRNA 2'-O-Methyltransferase Fibrillarin (FBL)

Autre désignation: NOP1

Sujet:

Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Gene Name: NOP1

ID gène: 851548

UniProt: P15646