BrdU anticorps

N° du produit ABIN669246

Détail du produit anti-BrdU anticorps

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Réactivité croisée: Humain, Rat

Réactivité croisée (Details): BrdU

Purification: Purified by Protein A.

Immunogène: BrdU conjugated to KLH

Isotype: IgG

Information d'application

Indications d'application: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-BrdU (ABIN669246)

Giuliani, Neri, Canalini, Calevro, Ottani, Vandini, Sena, Zaffe, Guarini: "NDP-?-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC4 receptors." dans: Molecular and cellular neurosciences, Vol. 67, pp. 13-21, (2015) (PubMed).

Chen, Li, Xu, Yin: "Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 34, Issue 4, pp. 1109-24, (2015) (PubMed).

Zhang, Zhang, Fang, Li, Zhao, Shi, An: "Neuroprotective effects of sulforaphane on cholinergic neurons in mice with Alzheimer's disease-like lesions." dans: International journal of molecular sciences, Vol. 15, Issue 8, pp. 14396-410, (2014) (PubMed).

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU (BrdU Produits)

Classe de substances: Chemical

Sujet:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis