Vimentin anticorps

N° du produit ABIN6699200

Détail du produit anti-Vimentin anticorps

Antigène: Vimentin (VIM)

Reactivité: Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat (Rattus)

Réactivité croisée (Details): Cross reactivity with Vimentin from other species has not been determined.

Purification: Anti-Vimentin antibody is directed against human Vimentin proteins. The antibody was purified from chicken eggs as an IgY fraction. Reactivity is expected from human, mouse and rat sources.

Immunogène:

Immunogen: Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Vimentin antibody is suitable for use in ELISA and Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appropriate cell lysate or extract.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin (VIM Produits)

Synonymes: anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet:

Synonyms: VIM

Background: Vimentin antibody recognizes Vimentin which is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin antibody is ideal for investigators involved in Neuroscience, Cell Migration and Cancer Research.

Gene Name: VIM

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis