PHF21A anticorps (AA 1-97)

N° du produit ABIN6719353

Cet anticorps Lapin Polyclonal détecte spécifiquement PHF21A dans WB, ELISA, IHC, IF et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PHF21A anticorps (AA 1-97) (ABIN6719353)

Antigène: PHF21A (PHD Finger Protein 21A (PHF21A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHF21A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-PHF21A anticorps

Épitope: AA 1-97

Fonction: Anti-BHC80/PHF21A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-BHC80/PHF21A Antibody (ABIN6719353). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347-55. 2. Iwase S, Januma A, Miyamoto K, Shono N, Honda A, Yanagisawa J, Baba T (Sep 2004). "Characterization of BHC80 in BRAF-HDAC complex, involved in neuron-specific gene repression". Biochem. Biophys. Res. Commun. 322 (2): 601-8.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PHF21A

Antigène: PHF21A (PHD Finger Protein 21A (PHF21A))

Autre désignation: PHF21A

Sujet:

Synonyms: PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696, BM-006

Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues.

Background: PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC, see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).

Poids moléculaire: 75-95 kDa

ID gène: 51317

Pathways: Feeding Behaviour