RAB5 anticorps (C-Term)

N° du produit ABIN6719366

L’anticorps Souris Monoclonal anti-RAB5 a été validé pour WB, IF, ICC et FACS. Il convient pour détecter RAB5 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour RAB5 anticorps (C-Term) (ABIN6719366)

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAB5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3E9

Détail du produit anti-RAB5 anticorps

Épitope: AA 179-215, C-Term

Fonction: Anti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9)

Séquence: KKLPKNEPQN PGANSARGRG VDLTEPTQPT RNQCCSN

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Rab5/RAB5A Antibody Picoband® (monoclonal, 3E9) (ABIN6719366). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1.Bucci, C., Parton, R. G., Mather, I. H., Stunnenberg, H., Simons, K., Hoflack, B., Zerial, M. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell 70: 715-728, 1992. 2.Kitano, M., Nakaya, M., Nakamura, T., Nagata, S., Matsuda, M. Imaging of Rab5 activity identifies essential regulators for phagosome maturation. Nature 453: 241-245, 2008.  3.Lanzetti, L., Rybin, V., Malabarba, M. G., Christoforidis, S., Scita, G., Zerial, M., Di Fiore, P. P. The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5. Nature 408: 374-377, 2000.

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur RAB5

Antigène: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Autre désignation: RAB5A

Sujet:

Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.

Poids moléculaire: 24 kDa

ID gène: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity