MID1 anticorps (AA 226-278)

N° du produit ABIN6719400

Détail du produit anti-MID1 anticorps

Antigène: MID1 (Midline 1 (MID1))

Épitope: AA 226-278

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MID1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for MID1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MID1 recombinant protein (Position: N226-Q278).

Isotype: IgG

Information d'application

Indications d'application:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur MID1

Antigène: MID1 (Midline 1 (MID1))

Autre désignation: MID1 (MID1 Produits)

Synonymes: anticorps BBBG1, anticorps FXY, anticorps GBBB1, anticorps MIDIN, anticorps OGS1, anticorps OS, anticorps OSX, anticorps RNF59, anticorps TRIM18, anticorps XPRF, anticorps ZNFXY, anticorps 61B3-R, anticorps DXHXS1141, anticorps Fxy, anticorps Trim18, anticorps Midline1, anticorps bbbg1, anticorps fxy, anticorps gbbb1, anticorps midin, anticorps ogs1, anticorps osx, anticorps rnf59, anticorps trim18, anticorps xprf, anticorps znfxy, anticorps midline 1, anticorps midline 1 L homeolog, anticorps MID1, anticorps Mid1, anticorps mid1, anticorps mid1.L

Sujet:

Synonyms: E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF

Background: Midline-1 is a protein found in humans that is encoded by the MID1 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities.

ID gène: 4281

UniProt: O15344