KRT8 anticorps (AA 107-325)

N° du produit ABIN6719478

Cet anticorps anti-KRT8 est un anticorps Souris Monoclonal détectant KRT8 dans WB, IHC, IF, FACS, ICC et IHC (fro). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour KRT8 anticorps (AA 107-325) (ABIN6719478)

Antigène: KRT8 (Keratin 8 (KRT8))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3G9

Détail du produit anti-KRT8 anticorps

Épitope: AA 107-325

Fonction: Anti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Cytokeratin 8 KRT8 Antibody Picoband® (monoclonal, 3G9) (ABIN6719478). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325). Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1.He, T., Stepulak, A., Holmstrom, T. H., Omary, M. B., Eriksson, J. E. : The intermediate filament protein kinase 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J. Biol. Chem. 277: 10767-10774, 2002. 2.Krauss, S., Franke, W. W. : Organization and sequence of the human gene encoding cytokeratin 8. Gene 86: 241-249, 1990. 3.Yamamoto, R., Kao, L.-C., McKnight, C. E., Strauss, J. F., III : Cloning and sequence of cDNA for human placental cytokeratin 8: regulation of the mRNA in trophoblastic cells by cAMP. Molec. Endocr. 4: 370-374, 1990.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur KRT8

Antigène: KRT8 (Keratin 8 (KRT8))

Autre désignation: KRT8

Sujet:

Synonyms: Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, K8, Type-II keratin Kb8, KRT8, CYK8

Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Background: Keratin, type II cytoskeletal 8, also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Poids moléculaire: 54 kDa

ID gène: 3856

UniProt: P05787