CHM anticorps (N-Term)

N° du produit ABIN6719537

L’anticorps anti-CHM Polyclonal Lapin est utilisé pour la détection de CHM dans des échantillons de Humain. Il a été validé pour WB, IF, ICC, IHC et FACS.

Aperçu rapide pour CHM anticorps (N-Term) (ABIN6719537)

Antigène: CHM (Choroideremia (Rab Escort Protein 1) (CHM))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHM est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-CHM anticorps

Épitope: N-Term

Fonction: Anti-CHM Antibody

Séquence: QDQILENEEA IALSRKDKTI QHVEVFCYAS QDLHED

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CHM Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human CHM, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat CHM.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CHM

Antigène: CHM (Choroideremia (Rab Escort Protein 1) (CHM))

Autre désignation: CHM

Sujet:

Background: Rab escort protein 1 (REP1) also known as rab proteins geranylgeranyltransferase component A 1 is an enzyme that in humans is encoded by the CHM gene. It is mapped to Xq21.2. This gene encodes component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Rab GTPases need to be geranylgeranyled on either one or two cysteine residues in their C-terminus to localize to the correct intracellular membrane. Mutations in this gene are a cause of choroideremia, also known as tapetochoroidal dystrophy (TCD). This X-linked disease is characterized by progressive dystrophy of the choroid, retinal pigment epithelium and retina. Alternatively spliced transcript variants have been found for this gene.

Gene Full Name: CHM Rab escort protein

Poids moléculaire: 100 kDa

ID gène: 1121

UniProt: P24386

Pathways: Sensory Perception of Sound