ADO anticorps (AA 49-261) (DyLight 550)

N° du produit ABIN7354747

L’anticorps Lapin Polyclonal anti-ADO a été validé pour FACS. Il convient pour détecter ADO dans des échantillons de Humain.

Aperçu rapide pour ADO anticorps (AA 49-261) (DyLight 550) (ABIN7354747)

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADO est conjugé à/à la DyLight 550

Application: Flow Cytometry (FACS)

Détail du produit anti-ADO anticorps (DyLight 550)

Épitope: AA 49-261

Fonction: Anti-Human ADO DyLight® 550 conjugated Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Human ADO DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.

Commentaires:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Détails sur ADO

Antigène: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Autre désignation: ADO

Sujet:

Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Poids moléculaire: 39 kDa