HMGN2 anticorps

N° du produit ABIN6719576

L’anticorps Lapin Polyclonal anti-HMGN2 a été validé pour IHC, WB, IF, ICC et FACS. Il convient pour détecter HMGN2 dans des échantillons de Humain.

Aperçu rapide pour HMGN2 anticorps (ABIN6719576)

Antigène: HMGN2 (High Mobility Group Nucleosomal Binding Domain 2 (HMGN2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HMGN2 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-HMGN2 anticorps

Fonction: Anti-HMGN2 Antibody Picoband®

Séquence: KKGKADAGKE GNNPAENGDA KTDQAQKAEG AGDAK

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-HMGN2 Antibody Picoband® (ABIN6719576). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human HMGN2, which shares 94.3% and 88.6% amino acid (aa) sequence identity with mouse and rat HMGN2, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Porkka, K., Laakkonen, P., Hoffman, J. A., Bernasconi, M., Ruoslahti, E. A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Proc. Nat. Acad. Sci. 99: 7444-7449, 2002. 2. West, K. L., Ito, Y., Birger, Y., Postnikov, Y., Shirakawa, H., Bustin, M.HMGN3a and HMGN3b, two protein isoforms with a tissue-specific expression pattern, expand the cellular repertoire of nucleosome-binding proteins. J. Biol. Chem. 276: 25959-25969, 2001.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur HMGN2

Antigène: HMGN2 (High Mobility Group Nucleosomal Binding Domain 2 (HMGN2))

Autre désignation: HMGN2

Sujet:

Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: Non-histone chromosomal protein HMG-17 is a protein that in humans is encoded by the HMGN2 gene. It is mapped to 1p36.11. The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.

Poids moléculaire: 18 kDa

ID gène: 3151

UniProt: P05204