HECTD3 anticorps

N° du produit ABIN6719595

Cet anticorps anti-HECTD3 est un anticorps Lapin Polyclonal détectant HECTD3 dans WB, IHC (fro), ICC, FACS et IHC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour HECTD3 anticorps (ABIN6719595)

Antigène: HECTD3 (HECT Domain Containing 3 (HECTD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HECTD3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Détail du produit anti-HECTD3 anticorps

Fonction: Anti-HECTD3 Antibody Picoband®

Séquence: HYASAKVCEE KLRYAAYNCV AIDTDMSPWE E

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-HECTD3 Antibody Picoband® (ABIN6719595). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human HECTD3, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, Human
1. Yu J., Lan J., Zhu Y., Li X., Lai X., Xue Y., Jin C., Huang H. The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara. Biochem. Biophys. Res. Commun. 367:805-812(2008)

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur HECTD3

Antigène: HECTD3 (HECT Domain Containing 3 (HECTD3))

Autre désignation: HECTD3

Sujet:

Synonyms: E3 ubiquitin-protein ligase HECTD3, HECT domain-containing protein 3, HECT-type E3 ubiquitin transferase HECTD3, HECTD3

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. This gene is mapped to 1p34.1. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8.

Poids moléculaire: 97 kDa

ID gène: 79654

UniProt: Q5T447