EHD1 anticorps (AA 324-516)

N° du produit ABIN6719599

Cet anticorps Lapin Polyclonal détecte spécifiquement EHD1 dans WB, ELISA, IHC, IF, ICC, FACS et IHC (fro). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour EHD1 anticorps (AA 324-516) (ABIN6719599)

Antigène: EHD1 (EH-Domain Containing 1 (EHD1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EHD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-EHD1 anticorps

Épitope: AA 324-516

Fonction: Anti-EHD1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EHD1 Antibody Picoband® (ABIN6719599). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human EHD1 recombinant protein (Position: K324-E516).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Haider, N. B., Searby, C., Galperin, E., Mintz, L., Horowitz, M., Stone, E. M., Sheffield, V. C. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1). Gene 240: 227-232, 1999. 2. Mintz, L., Galperin, E., Pasmanik-Chor, M., Tulzinsky, S., Bromberg, Y., Kozak, C. A., Joyner, A., Fein, A., Horowitz, M. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics 59: 66-76, 1999. 3. Naslavsky, N., Boehm, M., Backlund, P. S., Jr., Caplan, S. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molec. Biol. Cell 15: 2410-2422, 2004.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur EHD1

Antigène: EHD1 (EH-Domain Containing 1 (EHD1))

Autre désignation: EHD1

Sujet:

Synonyms: EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, CDABP0131

Tissue Specificity: Highly expressed in testis.

Background: EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13.1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.

Poids moléculaire: 61 kDa

ID gène: 10938

UniProt: Q9H4M9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development