DYKDDDDK Tag anticorps (DyLight 549)
Aperçu rapide pour DYKDDDDK Tag anticorps (DyLight 549) (ABIN6720684)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
N° du produit (Fournisseur)
- 200-342-383
-
Fournisseur
- Rockland
-
Fonction
- DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 549 Conjugated
-
Réactivité croisée (Details)
- The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
-
Purification
- This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.
-
Immunogène
- This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
-
Isotype
- IgG2a kappa
-
Labeling Ratio
- 2.1
-
-
-
-
Indications d'application
-
FLISA_Dilution: >1:20,000
ELISA_Dilution: 1:10,000 - 1:25,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
-
Commentaires
-
Anti-FLAG DyLight™549 has been tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Date de péremption
- 12 months
-
-
- DYKDDDDK Tag
-
Sujet
-
Synonyms: mouse anti-FLAG™ tag DyLight™549 conjugation, Enterokinase Cleavage Site (ECS), DyLight™549 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antigène
-