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DYKDDDDK Tag anticorps (DyLight 549)

Cet anticorps anti-DYKDDDDK Tag Monoclonal Souris conjugué à DyLight 549 (Clone 29E4-G7) (ABIN6720684) détecte spécifiquement DYKDDDDK Tag dans WB, ELISA, FM et FLISA.
Rockland
N° du produit ABIN6720684
N° du produit (Fournisseur): 200-342-383
562,24 €
Plus frais de livraison 40,00 € et TVA
100 μg
Destination: France
Envoi sous 5 à 8 jours ouvrables

Aperçu rapide pour DYKDDDDK Tag anticorps (DyLight 549) (ABIN6720684)

Antigène

DYKDDDDK Tag

Reactivité

Veuillez nous consulter SVP

Hôte

  • 91
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Souris

Clonalité

  • 103
  • 99
Monoclonal

Conjugué

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Cet anticorp DYKDDDDK Tag est conjugé à/à la DyLight 549

Application

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Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Clone

29E4-G7
  • N° du produit (Fournisseur)

    200-342-383

    Fournisseur

    Rockland

    Fonction

    DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 549 Conjugated

    Réactivité croisée (Details)

    The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

    Purification

    This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

    Immunogène

    This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

    Isotype

    IgG2a kappa

    Labeling Ratio

    2.1
  • Indications d'application

    FLISA_Dilution: >1:20,000

    ELISA_Dilution: 1:10,000 - 1:25,000

    IF_Microscopy_Dilution: >1:5,000

    Western_Blot_Dilution: >1:10,000

    Other: User Optimized

    Commentaires

    Anti-FLAG DyLight™549 has been tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    DYKDDDDK Tag

    Sujet

    Synonyms: mouse anti-FLAG™ tag DyLight™549 conjugation, Enterokinase Cleavage Site (ECS), DyLight™549 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

    Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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