GFP anticorps (DyLight 549)

N° du produit ABIN6720702

N° du produit (Fournisseur): 200-342-215

Cet anticorps anti-GFP est un anticorps Souris Monoclonal détectant GFP dans WB, FM et FLISA. Adapté pour Aequorea victoria.

Aperçu rapide pour GFP anticorps (DyLight 549) (ABIN6720702)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est conjugé à/à la DyLight 549

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Clone: 9F9-F9

Détail du produit anti-GFP anticorps (DyLight 549)

N° du produit (Fournisseur): 200-342-215

Fournisseur: Rockland

Fonction: GFP Antibody Dylight™ 549 Conjugated

Specificité: Wt, rGFP, eGFP

Réactivité croisée (Details): Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

Purification: GFP Dylight™ 549 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

Immunogène: BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.

Isotype: IgG1 kappa

Labeling Ratio: 4.0

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Commentaires:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet:

Synonyms: mouse anti-GFP Antibody Dylight™ 549 Conjugation, Dylight™ 549 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP monoclonal antibody is Dylight™ 549 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.

UniProt: P42212