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GST anticorps (DyLight 680)

L’anticorps Lapin Polyclonal anti-GST a été validé pour WB, FLISA et FM. Il convient pour détecter GST dans des échantillons de Schistosoma japonicum. Il y a 1 publication disponible.
Rockland
N° du produit ABIN6720737
N° du produit (Fournisseur): 600-444-200

Aperçu rapide pour GST anticorps (DyLight 680) (ABIN6720737)

Antigène

Voir toutes GST Anticorps
GST (Glutathione S Transferase (GST))

Reactivité

  • 96
  • 29
  • 14
  • 9
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  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Schistosoma japonicum

Hôte

  • 64
  • 52
  • 49
  • 2
  • 1
  • 1
Lapin

Clonalité

  • 99
  • 68
Polyclonal

Conjugué

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Cet anticorp GST est conjugé à/à la DyLight 680

Application

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Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
  • N° du produit (Fournisseur)

    600-444-200

    Fournisseur

    Rockland

    Fonction

    GST Antibody Dylight™ 680 Conjugated

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

    Immunogène

    The immunogen is full length GST isolated from Schistosoma japonicum.

    Isotype

    IgG

    Labeling Ratio

    2.9
  • Indications d'application

    FLISA_Dilution: >1:10,000

    IF_Microscopy_Dilution: >1:5,000

    Other: User Optimized

    Commentaires

    Anti-GST DyLight 680 Conjugated Antibody has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Waizenegger, Urulangodi, Lehmann, Reyes, Saugar, Tercero, Szakal, Branzei: "Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity." dans: Nature communications, Vol. 11, Issue 1, pp. 5746, (2020) (PubMed).

  • Antigène

    GST (Glutathione S Transferase (GST))

    Autre désignation

    GST

    Sujet

    Synonyms: rabbit anti-GST antibody DyLight™680 conjugation, DyLight™680 conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

    Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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