GST anticorps (DyLight 680)

N° du produit ABIN6720737

Détail du produit anti-GST anticorps (DyLight 680)

Antigène: GST (Glutathione S Transferase (GST))

Reactivité: Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GST est conjugé à/à la DyLight 680

Application: FLISA, Fluorescence Microscopy (FM)

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Immunogène:

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2.9

Information d'application

Indications d'application:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Détails sur GST

Antigène: GST (Glutathione S Transferase (GST))

Autre désignation: GST (GST Produits)

Synonymes: anticorps GST, anticorps GST 13-13, anticorps GST13, anticorps GST13-13, anticorps GSTK1-1, anticorps hGSTK1, anticorps 1500002K10Rik, anticorps Gst, anticorps Ptgds2, anticorps glutathione S-transferase kappa 1, anticorps microsomal glutathione S-transferase 1, anticorps hematopoietic prostaglandin D synthase, anticorps GSTK1, anticorps Mgst1, anticorps Hpgds

Sujet:

Synonyms: rabbit anti-GST antibody DyLight™680 conjugation, DyLight™680 conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.