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Recombinant Rpb1 CTD anticorps (pTyr1)

L’anticorps Souris Monoclonal anti-Rpb1 CTD a été validé pour WB, IF et ELISA. Il convient pour détecter Rpb1 CTD dans des échantillons de Humain.
N° du produit ABIN6731014

Aperçu rapide pour Recombinant Rpb1 CTD anticorps (pTyr1) (ABIN6731014)

Antigène

Rpb1 CTD (RNA Polymerase II CTD)

Type d'anticorp

Recombinant Antibody

Reactivité

  • 24
  • 7
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 11
  • 9
  • 4
Souris

Clonalité

  • 15
  • 9
Monoclonal

Conjugué

  • 24
Cet anticorp Rpb1 CTD est non-conjugé

Application

  • 24
  • 17
  • 15
  • 10
  • 7
  • 4
  • 3
  • 3
  • 2
  • 1
Western Blotting (WB), Immunofluorescence (IF), ELISA
  • Épitope

    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    pTyr1

    Marque

    AbFlex®

    Purification

    Protein A Chromatography

    Immunogène

    This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

    Isotype

    IgG2a
  • Indications d'application


    Validated Applications:
    IF: 0.5 - 2 µg/ml
    Bead-based ELISA: 0.5 - 10 µg/ml
    WB: 1 - 2 μg/mL

    For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required.

    Commentaires

    AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C
  • Antigène

    Rpb1 CTD (RNA Polymerase II CTD)

    Autre désignation

    RNA Pol II CTD

    Poids moléculaire

    210 kDa
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