Recombinant Rpb1 CTD anticorps (pTyr1)
Aperçu rapide pour Recombinant Rpb1 CTD anticorps (pTyr1) (ABIN6731014)
Antigène
Type d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pTyr1
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Marque
- AbFlex®
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Purification
- Protein A Chromatography
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Immunogène
- This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
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Isotype
- IgG2a
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Indications d'application
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Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL
For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required. -
Commentaires
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C
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- Rpb1 CTD (RNA Polymerase II CTD)
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Autre désignation
- RNA Pol II CTD
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Poids moléculaire
- 210 kDa
Antigène
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