GLIS1 anticorps

N° du produit ABIN7938140

Cet anticorps Rat Monoclonal détecte spécifiquement GLIS1 dans WB et IF. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour GLIS1 anticorps (ABIN7938140)

Antigène: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GLIS1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 7-14

Détail du produit anti-GLIS1 anticorps

Fonction: GLIS1 antibody (mAb) (Clone 7-14)

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a peptide within the amino-terminal half of human GLIS1 (GLI-similar 1), which shares homology with mouse GLIS1.

Isotype: IgG1 kappa

Information d'application

Indications d'application: WB*: 0.5 - 2 μg/mL IF: 1-2 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur GLIS1

Antigène: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Autre désignation: GLIS1

Sujet: GLIS1 (Glis Family Zinc Finger 1) belongs to a GLIS subfamily of Krüppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, b-cell maturation and maintenance, and insulin production. GLIS1 is enriched in unfertilized eggs and embryos and it can be used to promote direct reprogramming of somatic cells to induced pluripotent stem cells (iPS cells). GLIS1 may regulate the expression of numerous genes, either positively or negatively, and is implicated in mitral valve development, where dysregulation may lead to mitral valve prolapse (MVP).

Poids moléculaire: 95 kDa

NCBI Accession: NP_671726