Recombinant Histone H4 anticorps (meLys20)

N° du produit ABIN6731025

Cet anticorps Souris Monoclonal détecte spécifiquement Histone H4 dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Histone H4 anticorps (meLys20) (ABIN6731025)

Antigène: Histone H4

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Histone H4 anticorps

Épitope: meLys20

Marque: AbFlex®

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

Isotype: IgG2a

Information d'application

Indications d'application:
Validated Applications:
WB: 0.5 - 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet; therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Commentaires:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Histone H4

Antigène: Histone H4

Poids moléculaire: 12 kDa