Recombinant PIWIL2 anticorps (Sortase,His tag,AVI tag)

N° du produit ABIN6731041

L’anticorps anti-PIWIL2 Monoclonal Souris est utilisé pour la détection de PIWIL2 dans des échantillons de Souris. Il a été validé pour WB.

Aperçu rapide pour Recombinant PIWIL2 anticorps (Sortase,His tag,AVI tag) (ABIN6731041)

Antigène: PIWIL2 (Piwi-Like 2 (PIWIL2))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PIWIL2 est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB)

Détail du produit anti-PIWIL2 anticorps (Sortase,His tag,AVI tag)

Fonction: AbFlex Mili / PiwiL2 antibody (rAb)

Marque: AbFlex®

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against full length mouse MILI / PIWIL2 (Miwi like / piwi-like 2) recombinant protein.

Isotype: IgG2a

Information d'application

Indications d'application: WB: 0.2 - 2 μg/mL AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61143.

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur PIWIL2

Antigène: PIWIL2 (Piwi-Like 2 (PIWIL2))

Autre désignation: Mili / PiwiL2

Sujet: AbFlex Mili / PiwiL2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

Poids moléculaire: 110 kDa

NCBI Accession: NP_001129193

Pathways: Stem Cell Maintenance