ESR2 anticorps (AA 82-131)

N° du produit ABIN214750

Cet anticorps Lapin Polyclonal détecte spécifiquement ESR2 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Porc, Boeuf (Vache), Lapin et Roussette (Chauve-souris).

Aperçu rapide pour ESR2 anticorps (AA 82-131) (ABIN214750)

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Lapin, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ESR2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ESR2 anticorps

Épitope: AA 82-131

Marque: IHC-plus™

Specificité: Human ESR2 / ER-Beta

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Sheep, Dog, Guinea pig (92%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa82-131 of human ESR2 (Q92731, NP_001428). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Bovine, Bat, Pig (100%), Galago, Marmoset, Sheep, Panda, Dog, Guinea pig (92%), Hamster, Elephant, Cat (85%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with sterile water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ESR2

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: ESR2 / ER Beta

Sujet: Name/Gene ID: ESR2
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

ID gène: 2100

NCBI Accession: NP_001428

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling