PHD1 anticorps (AA 58-107)

N° du produit ABIN6736715

L’anticorps Lapin Polyclonal anti-PHD1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter PHD1 dans des échantillons de Humain, Boeuf (Vache) et Cobaye.

Aperçu rapide pour PHD1 anticorps (AA 58-107) (ABIN6736715)

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivité: Humain, Boeuf (Vache), Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PHD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PHD1 anticorps

Épitope: AA 58-107

Marque: IHC-plus™

Specificité: Human EGLN2 / PHD1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Guinea pig (85%).

Purification: Protein A purified

Immunogène: Synthetic peptide located between aa58-107 of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse, Rat, Dog, Pig (92%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PHD1

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Autre désignation: EGLN2 / PHD1

Sujet: Name/Gene ID: EGLN2

Synonyms: EGLN2, EIT6, EGL nine (C.elegans) homolog 2, Egl nine homolog 2, HPH-1, HPH-3, Estrogen-induced tag 6, PHD1, HIF-PH1, HIF-prolyl hydroxylase 1, HIFPH1

ID gène: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis