His Tag anticorps (HRP)
Aperçu rapide pour His Tag anticorps (HRP) (ABIN6737087)
Antigène
Voir toutes His Tag AnticorpsHôte
Clonalité
Conjugué
Application
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Séquence
- HHHHHH
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Specificité
- Recognizes native and denatured-reduced forms of synthetic poly-histidine or poly-histidine-tagged fusion proteins. Recognizes fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
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Purification
- Immunoaffinity purified
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Immunogène
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His-tag fusion protein.
Type of Immunogen: Fusion protein -
Isotype
- IgG2a
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Indications d'application
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Approved: ELISA (1:10000 - 1:50000), IHC (1:200 - 1:2000), WB (1:10000)
Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Western Blot: 1:10000. ELISA: 1:10000-1:50000 using 50-100 ng control antigen/well. Immunohistochemistry: 1:200-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 0.05 % merthiolate
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Agent conservateur
- Thimerosal (Merthiolate)
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Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- avoid freeze thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
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- His Tag
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Classe de substances
- Tag
Antigène
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