EN2 anticorps (C-Term)

N° du produit ABIN6741528

L’anticorps Lapin Polyclonal anti-EN2 a été validé pour WB, IHC et IHC (p). Il convient pour détecter EN2 dans des échantillons de Humain, Souris, Rat, Cobaye, Cheval, Singe et Porc.

Aperçu rapide pour EN2 anticorps (C-Term) (ABIN6741528)

Antigène: EN2 (Engrailed Homeobox 2 (EN2))

Reactivité: Humain, Souris, Rat, Cobaye, Cheval, Singe, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EN2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EN2 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human EN2

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Horse, Guinea pig (100%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human EN2 (P19622, NP_001418). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Horse, Pig, Guinea pig (100%), Bat, Opossum, Zebra finch, Chicken, Platypus (92%), Xenopus, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.05 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur EN2

Antigène: EN2 (Engrailed Homeobox 2 (EN2))

Autre désignation: EN2

Sujet: Name/Gene ID: EN2

Synonyms: EN2, AUTS10, Engrailed homolog 2, Engrailed homeobox 2, Homeobox protein engrailed-2, Engrailed-2, Homeobox protein en-2, Hu-En-2

ID gène: 2020

NCBI Accession: NP_001418

UniProt: P19622