GATA3 anticorps (AA 36-85)

N° du produit ABIN462169

Cet anticorps Lapin Polyclonal détecte spécifiquement GATA3 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Porc, Poisson zèbre (Danio rerio), Chien, Lapin, Poulet, Cheval, Mouton, Singe et Hamster.

Aperçu rapide pour GATA3 anticorps (AA 36-85) (ABIN462169)

Antigène: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Poisson zèbre (Danio rerio), Chien, Lapin, Poulet, Cheval, Mouton, Singe, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GATA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GATA3 anticorps

Épitope: AA 36-85

Specificité: Human GATA3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bat, Guinea pig, Trout, Salmon (92%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur GATA3

Antigène: GATA3 (GATA Binding Protein 3 (GATA3))

Autre désignation: GATA3

Sujet: Name/Gene ID: GATA3
Family: Transcription factor

Synonyms: GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR

ID gène: 2625

NCBI Accession: NP_002042

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation