FHL1 anticorps (AA 51-100)

N° du produit ABIN6741535

Cet anticorps anti-FHL1 est un anticorps Lapin Polyclonal détectant FHL1 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Mouton, Lapin, Porc et Hamster.

Aperçu rapide pour FHL1 anticorps (AA 51-100) (ABIN6741535)

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Mouton, Lapin, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FHL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-FHL1 anticorps

Épitope: AA 51-100

Marque: IHC-plus™

Specificité: Human FHL1

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Horse (92%) Human, Xenopus (91%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa51-100 of human FHL1 (Q6IB30, NP_001440). Percent identity by BLAST analysis: Human, Chimpanzee, Galago, Mouse, Rat, Sheep, Hamster, Dog, Bovine, Rabbit, Pig, Guinea pig (100%), Horse, Platypus, Xenopus (92%), Gorilla, Orangutan, Gibbon, Monkey, Marmoset (91%), Elephant, Turkey, Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur FHL1

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Autre désignation: SLIM / FHL1

Sujet: Name/Gene ID: FHL1

Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1

ID gène: 2273

NCBI Accession: NP_001440

UniProt: Q13642