NCOA3 anticorps (AA 576-625)

N° du produit ABIN6741542

L’anticorps Lapin Polyclonal anti-NCOA3 a été validé pour WB, IHC et IHC (p). Il convient pour détecter NCOA3 dans des échantillons de Humain, Boeuf (Vache), Porc et Singe.

Aperçu rapide pour NCOA3 anticorps (AA 576-625) (ABIN6741542)

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivité: Humain, Boeuf (Vache), Porc, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCOA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NCOA3 anticorps

Épitope: AA 576-625

Marque: IHC-plus™

Specificité: Human NCOA3

Homologie: Percent identity by BLAST analysis: Human (100%) Horse, Pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa576-625 of human NCOA3 (Q0VF45, NP_006525). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Pig (100%), Horse (92%), Marmoset, Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NCOA3

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Autre désignation: NCOA3 / SRC-3 / AIB1

Sujet: Name/Gene ID: NCOA3

Synonyms: NCOA3, ACTR, AIB1, BHLHe42, CAGH16, CBP-interacting protein, CTG26, AIB-1, P/CIP, TNRC14, TRAM-1, Nuclear receptor coactivator 3, PCIP, RAC-3, SRC3, TNRC16, KAT13B, NCoA-3, SRC-3

ID gène: 8202

NCBI Accession: NP_006525

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha