Ceramide Synthase 2 anticorps (AA 72-121)

N° du produit ABIN6741543

Cet anticorps Lapin Polyclonal détecte spécifiquement Ceramide Synthase 2 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Cobaye, Porc et Hamster.

Aperçu rapide pour Ceramide Synthase 2 anticorps (AA 72-121) (ABIN6741543)

Antigène: Ceramide Synthase 2 (CERS2)

Reactivité: Humain, Cobaye, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ceramide Synthase 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Ceramide Synthase 2 anticorps

Épitope: AA 72-121

Marque: IHC-plus™

Specificité: Human LASS2

Homologie: Percent identity by BLAST analysis: Pig (100%) Mouse, Horse (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa72-121 of human CERS2 (Q96G23, NP_071358). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Hamster, Pig, Guinea pig (100%), Orangutan, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Opossum (92%), Bovine, Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Ceramide Synthase 2

Antigène: Ceramide Synthase 2 (CERS2)

Autre désignation: L3 / LASS2

Sujet: Name/Gene ID: CERS2

Synonyms: CERS2, Ceramide synthase 2, LAG1 longevity assurance 2, L3, LASS2, SP260, TMSG1

ID gène: 29956

NCBI Accession: NP_071358

UniProt: Q96G23