Sp3 anticorps (AA 287-336)

N° du produit ABIN6741550

Détail du produit anti-Sp3 anticorps

Antigène: Sp3 (Sp3 Transcription Factor (Sp3))

Épitope: AA 287-336

Reactivité: Humain, Souris, Rat, Cobaye, Cheval, Lapin, Boeuf (Vache), Chien, Roussette (Chauve-souris), Singe, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Sp3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human SP3

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig (100%) Chicken (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa287-336 of human SP3 (Q02447, NP_003102). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Chicken (92%), Turkey, Zebra finch, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Sp3

Antigène: Sp3 (Sp3 Transcription Factor (Sp3))

Autre désignation: SP3 (Sp3 Produits)

Synonymes: anticorps SPR2, anticorps D130027J01Rik, anticorps SP3, anticorps sp3, anticorps zgc:162922, anticorps Sp3 transcription factor, anticorps trans-acting transcription factor 3, anticorps sp3a transcription factor, anticorps Sp3 transcription factor S homeolog, anticorps SP3, anticorps Sp3, anticorps sp3a, anticorps sp3.S

Sujet: Name/Gene ID: SP3

Synonyms: SP3, SPR2, Transcription factor Sp3, Sp3 transcription factor, SPR-2, Specificity protein 3

ID gène: 6670

NCBI Accession: NP_003102

UniProt: Q02447