ASH2L anticorps (AA 288-337)

N° du produit ABIN6741556

L’anticorps Lapin Polyclonal anti-ASH2L a été validé pour WB, IHC et IHC (p). Il convient pour détecter ASH2L dans des échantillons de Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Singe, Roussette (Chauve-souris), Lapin, Porc et Hamster.

Aperçu rapide pour ASH2L anticorps (AA 288-337) (ABIN6741556)

Antigène: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reactivité: Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Singe, Roussette (Chauve-souris), Lapin, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASH2L est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ASH2L anticorps

Épitope: AA 288-337

Marque: IHC-plus™

Specificité: Human ASH2L

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%) Bovine, Chicken (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa288-337 of human ASH2L (Q9UBL3, NP_004665). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%), Bovine, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ASH2L

Antigène: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Autre désignation: ASH2L / ASH2

Sujet: Name/Gene ID: ASH2L

Synonyms: ASH2L, ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2

ID gène: 9070

NCBI Accession: NP_004665

UniProt: Q9UBL3