TRIM35 anticorps (AA 359-408)

N° du produit ABIN6741557

Cet anticorps anti-TRIM35 est un anticorps Lapin Polyclonal détectant TRIM35 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Lapin, Chien et Porc.

Aperçu rapide pour TRIM35 anticorps (AA 359-408) (ABIN6741557)

Antigène: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Lapin, Chien, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM35 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRIM35 anticorps

Épitope: AA 359-408

Marque: IHC-plus™

Specificité: Human TRIM35

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig (100%) Rabbit (92%) Rat (91%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa359-408 of human TRIM35 (Q9UPQ4, NP_741983). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Marmoset, Bat (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TRIM35

Antigène: TRIM35 (Tripartite Motif Containing 35 (TRIM35))

Autre désignation: TRIM35

Sujet: Name/Gene ID: TRIM35
Family: Tripartite Motif

Synonyms: TRIM35, HLS5, KIAA1098, MAIR, Tripartite motif containing 35, Tripartite motif-containing 35

ID gène: 23087

NCBI Accession: NP_741983

UniProt: Q9UPQ4