NRF1 anticorps (AA 360-409)

N° du produit ABIN462197

L’anticorps Lapin Polyclonal anti-NRF1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter NRF1 dans des échantillons de Humain, Rat, Boeuf (Vache), Lapin, Cheval, Cobaye, Poisson zèbre (Danio rerio), Chien, Singe, Poulet, Porc et Xenopus laevis.

Aperçu rapide pour NRF1 anticorps (AA 360-409) (ABIN462197)

Antigène: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivité: Humain, Rat, Boeuf (Vache), Lapin, Cheval, Cobaye, Poisson zèbre (Danio rerio), Chien, Singe, Poulet, Porc, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NRF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NRF1 anticorps

Épitope: AA 360-409

Marque: IHC-plus™

Specificité: Human NRF1

Aucune reactivité croisée: Souris

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NRF1

Antigène: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Autre désignation: NRF1 / NRF-1

Sujet: Name/Gene ID: NRF1
Family: Transcription factor

Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL

ID gène: 4899

NCBI Accession: NP_005002

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha