SSX4 anticorps (AA 108-157)

N° du produit ABIN462200

L’anticorps Lapin Polyclonal anti-SSX4 a été validé pour WB, IHC et IHC (p). Il convient pour détecter SSX4 dans des échantillons de Humain.

Aperçu rapide pour SSX4 anticorps (AA 108-157) (ABIN462200)

Antigène: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SSX4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SSX4 anticorps

Épitope: AA 108-157

Specificité: Human SSX4

Homologie: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa108-157 of human SSX4 (O60224, NP_005627). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (85%), Gibbon (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur SSX4

Antigène: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Autre désignation: SSX4

Sujet: Name/Gene ID: SSX4

Synonyms: SSX4, CT5.4, Cancer/testis antigen 5.4, Protein SSX4, SSX4A, SSX4B

ID gène: 6759

NCBI Accession: NP_005627