RHOXF2 anticorps (AA 108-157)

N° du produit ABIN6741566

Détail du produit anti-RHOXF2 anticorps

Antigène: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Épitope: AA 108-157

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RHOXF2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human RHOXF2

Homologie: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa108-157 of human RHOXF2 (Q9BQY4, NP_115887). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%), Monkey (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur RHOXF2

Antigène: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Autre désignation: RHOXF2 (RHOXF2 Produits)

Synonymes: anticorps CT107, anticorps PEPP-2, anticorps PEPP2, anticorps THG1, anticorps Rhox homeobox family member 2, anticorps RHOXF2

Sujet: Name/Gene ID: RHOXF2

Synonyms: RHOXF2, Cancer/testis antigen 107, Homeobox protein from AL590526, PEPP subfamily gene 2, PEPP2, PEPP-2, Rhox homeobox family, member 2, Rhox homeobox family member 2, THG1, Testis homeobox gene 1, CT107

ID gène: 84528

NCBI Accession: NP_115887

UniProt: Q9BQY4