SP4 anticorps (C-Term)

N° du produit ABIN6741568

Cet anticorps anti-SP4 est un anticorps Lapin Polyclonal détectant SP4 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Cheval, Lapin, Boeuf (Vache), Chien, Singe, Porc, Poulet et Xenopus laevis.

Aperçu rapide pour SP4 anticorps (C-Term) (ABIN6741568)

Antigène: SP4 (Sp4 Transcription Factor (SP4))

Reactivité: Humain, Souris, Rat, Cheval, Lapin, Boeuf (Vache), Chien, Singe, Porc, Poulet, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SP4 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human SP4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human SP4 (Q02446, NP_003103). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur SP4

Antigène: SP4 (Sp4 Transcription Factor (SP4))

Autre désignation: SP4

Sujet: Name/Gene ID: SP4

Synonyms: SP4, HF1B, Sp4 transcription factor, SPR-1, Transcription factor Sp4

ID gène: 6671

NCBI Accession: NP_003103

UniProt: Q02446