NKRF anticorps (C-Term)

N° du produit ABIN6741576

Détail du produit anti-NKRF anticorps

Antigène: NKRF (NFKB Repressing Factor (NKRF))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Chien, Cheval, Lapin, Boeuf (Vache), Cobaye, Porc, Singe, Roussette (Chauve-souris), Hamster, Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NKRF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NKRF

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human NKRF (O15226, NP_060014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NKRF

Antigène: NKRF (NFKB Repressing Factor (NKRF))

Autre désignation: NKRF / NRF (NKRF Produits)

Synonymes: anticorps NKRF, anticorps NRF, anticorps nrf, anticorps itba4, anticorps 9430034D17Rik, anticorps AW124770, anticorps C80287, anticorps ITBA4, anticorps RGD1563950, anticorps NFKB repressing factor, anticorps NFKB repressing factor L homeolog, anticorps NF-kappaB repressing factor, anticorps NFKB repressing factor-like, anticorps NKRF, anticorps nkrf, anticorps nkrf.L, anticorps Nkrf, anticorps Nkrfl

Sujet: Name/Gene ID: NKRF

Synonyms: NKRF, NFkB-repressing factor, ITBA4, Protein ITBA4, NF-kappaB repressing factor, NFKB repressing factor, Transcription factor NRF, NF-kappa-B-repressing factor, NRF

ID gène: 55922

NCBI Accession: NP_060014

UniProt: O15226