BCL11A anticorps (N-Term)

N° du produit ABIN6741578

Cet anticorps anti-BCL11A est un anticorps Lapin Polyclonal détectant BCL11A dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Poisson zèbre (Danio rerio), Poulet, Roussette (Chauve-souris) et Singe.

Aperçu rapide pour BCL11A anticorps (N-Term) (ABIN6741578)

Antigène: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Poisson zèbre (Danio rerio), Poulet, Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BCL11A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BCL11A anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human BCL11A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Chicken, Zebrafish (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human BCL11A (Q9H165, NP_075044). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Chicken, Platypus, Zebrafish (100%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur BCL11A

Antigène: BCL11A (B-Cell CLL/lymphoma 11A (Zinc Finger Protein) (BCL11A))

Autre désignation: BCL11A

Sujet: Name/Gene ID: BCL11A
Family: Cancer

Synonyms: BCL11A, B-cell CLL/lymphoma 11A, BCL11A-L, BCL11A-XL, BCL11a-M, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A-S, CTIP1, COUP-TF-interacting protein 1, HBFQTL5, EVI-9, KIAA1809, ZNF856, C2H2-type zinc finger protein, EVI9, Zinc finger protein 856

ID gène: 53335

NCBI Accession: NP_075044

UniProt: Q9H165

Pathways: Regulation of Cell Size