TRIM8 anticorps (AA 36-85)

N° du produit ABIN6741584

Cet anticorps anti-TRIM8 est un anticorps Lapin Polyclonal détectant TRIM8 dans WB, IHC et IHC (p). Adapté pour Humain, Rat, Souris, Chien, Boeuf (Vache), Lapin, Porc, Poisson zèbre (Danio rerio), Hamster et Singe.

Aperçu rapide pour TRIM8 anticorps (AA 36-85) (ABIN6741584)

Antigène: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache), Lapin, Porc, Poisson zèbre (Danio rerio), Hamster, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRIM8 anticorps

Épitope: AA 36-85

Marque: IHC-plus™

Specificité: Human TRIM8 / GERP

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur TRIM8

Antigène: TRIM8 (Tripartite Motif Containing 8 (TRIM8))

Autre désignation: TRIM8 / GERP

Sujet: Name/Gene ID: TRIM8
Family: Tripartite Motif

Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8

ID gène: 81603

NCBI Accession: NP_112174

UniProt: Q9BZR9