POLDIP3 anticorps (AA 326-375)

N° du produit ABIN6741591

Cet anticorps Lapin Polyclonal détecte spécifiquement POLDIP3 dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Cobaye, Lapin, Singe, Roussette (Chauve-souris) et Porc.

Aperçu rapide pour POLDIP3 anticorps (AA 326-375) (ABIN6741591)

Antigène: POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Cobaye, Lapin, Singe, Roussette (Chauve-souris), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLDIP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLDIP3 anticorps

Épitope: AA 326-375

Marque: IHC-plus™

Specificité: Human POLDIP3 / SKAR

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa326-375 of human POLDIP3 (Q9BY77, NP_115687). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus (85%), Sea squirt (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur POLDIP3

Antigène: POLDIP3 (Polymerase (DNA-Directed), delta Interacting Protein 3 (POLDIP3))

Autre désignation: POLDIP3 / p46

Sujet: Name/Gene ID: POLDIP3

Synonyms: POLDIP3, p46, KIAA1649, SKAR, RNA-binding protein P46, PDIP46, S6K1 Aly/REF-like target

ID gène: 84271

NCBI Accession: NP_115687

UniProt: Q9BY77