NPY1R anticorps (AA 180-229)

N° du produit ABIN462232

Cet anticorps Lapin Polyclonal détecte spécifiquement NPY1R dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Cheval et Singe.

Aperçu rapide pour NPY1R anticorps (AA 180-229) (ABIN462232)

Antigène: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Reactivité: Humain, Souris, Rat, Cheval, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NPY1R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NPY1R anticorps

Épitope: AA 180-229

Marque: IHC-plus™

Specificité: Human NPY1R

Homologie: Percent identity by BLAST analysis: Human (100%) Sheep, Dog, Bovine, Rabbit, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa180-229 of human NPY1R (P25929, NP_000900). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Sheep, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig (92%), Hamster (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NPY1R

Antigène: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Autre désignation: NPY1R

Sujet: Name/Gene ID: NPY1R
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPY1R, NPYY1, NPY-Y1 receptor, Neuropeptide Y receptor type 1, PY receptor Y1, Neuropeptide Y receptor Y1, Neuropeptide Y-Y1 receptor, Neuropeptide Y1 receptor, NPY1-R, NPYR

ID gène: 4886

NCBI Accession: NP_000900

UniProt: P25929

Pathways: Negative Regulation of Hormone Secretion, Feeding Behaviour