GTPBP10 anticorps (AA 254-303)

N° du produit ABIN6741598

Cet anticorps anti-GTPBP10 est un anticorps Lapin Polyclonal détectant GTPBP10 dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Lapin, Cheval, Porc, Hamster et Singe.

Aperçu rapide pour GTPBP10 anticorps (AA 254-303) (ABIN6741598)

Antigène: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Reactivité: Humain, Souris, Rat, Lapin, Cheval, Porc, Hamster, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GTPBP10 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GTPBP10 anticorps

Épitope: AA 254-303

Marque: IHC-plus™

Specificité: Human GTPBP10

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa254-303 of human GTPBP10 (A4D1E9, NP_149098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Rabbit, Horse, Pig, Opossum, Armadillo, Lizard, Stickleback (100%), Dog, Bovine, Bat, Guinea pig (92%), Salmon (90%), Turkey, Zebra finch, Chicken, Platypus (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur GTPBP10

Antigène: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Autre désignation: GTPBP10

Sujet: Name/Gene ID: GTPBP10

Synonyms: GTPBP10, DKFZP686A10121, ObgH2, Protein obg homolog 2, UG0751c10, GTP-binding protein 10

ID gène: 85865

NCBI Accession: NP_149098

UniProt: A4D1E9