MED4 anticorps (AA 146-195)

N° du produit ABIN6741609

L’anticorps Lapin Polyclonal anti-MED4 a été validé pour WB, IHC et IHC (p). Il convient pour détecter MED4 dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Chien, Cobaye, Cheval, Lapin, Poulet, Singe, Xenopus laevis et Porc.

Aperçu rapide pour MED4 anticorps (AA 146-195) (ABIN6741609)

Antigène: MED4 (Mediator Complex Subunit 4 (MED4))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poisson zèbre (Danio rerio), Chien, Cobaye, Cheval, Lapin, Poulet, Singe, Xenopus laevis, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MED4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MED4 anticorps

Épitope: AA 146-195

Marque: IHC-plus™

Specificité: Human MED4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa146-195 of human MED4 (Q9NPJ6, NP_054885). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Stickleback, Pike, Zebrafish (100%), Drosophila, Mosquito (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur MED4

Antigène: MED4 (Mediator Complex Subunit 4 (MED4))

Autre désignation: MED4

Sujet: Name/Gene ID: MED4

Synonyms: MED4, ARC36, DRIP36, HSPC126, Mediator complex subunit 4, TRAP/SMCC/PC2 subunit p36, TRAP36, VDRIP

ID gène: 29079

NCBI Accession: NP_054885

UniProt: Q9NPJ6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha