ARID5A anticorps (C-Term)

N° du produit ABIN6741610

Cet anticorps anti-ARID5A est un anticorps Lapin Polyclonal détectant ARID5A dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Chien, Cobaye, Lapin et Singe.

Aperçu rapide pour ARID5A anticorps (C-Term) (ABIN6741610)

Antigène: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Lapin, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ARID5A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ARID5A anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human ARID5A

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur ARID5A

Antigène: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Autre désignation: MRF-1 / ARID5A

Sujet: Name/Gene ID: ARID5A

Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I

ID gène: 10865

NCBI Accession: NP_997646

UniProt: Q03989