Desmoglein 2 anticorps (AA 72-121)

N° du produit ABIN6744433

L’anticorps Lapin Polyclonal anti-Desmoglein 2 a été validé pour WB, IHC et IHC (p). Il convient pour détecter Desmoglein 2 dans des échantillons de Humain, Rat, Souris et Singe.

Aperçu rapide pour Desmoglein 2 anticorps (AA 72-121) (ABIN6744433)

Antigène: Desmoglein 2 (DSG2)

Reactivité: Humain, Rat, Souris, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Desmoglein 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Desmoglein 2 anticorps

Épitope: AA 72-121

Marque: IHC-plus™

Specificité: Human DSG2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Rabbit, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa72-121 of human DSG2 (Q14126, NP_001934). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat (100%), Elephant, Bovine, Rabbit, Horse, Guinea pig (92%), Bat (85%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Desmoglein 2

Antigène: Desmoglein 2 (DSG2)

Autre désignation: DSG2 / Desmoglein 2

Sujet: Name/Gene ID: DSG2
Family: Cadherin

Synonyms: DSG2, ARVC10, Cadherin family member 5, CDHF5, CMD1BB, Desmoglein-2, Desmoglein II, HDGC, ARVD10, Desmoglein 2

ID gène: 1829

NCBI Accession: NP_001934

UniProt: Q14126

Pathways: Cell-Cell Junction Organization