GNAS anticorps (AA 36-85)

N° du produit ABIN490056

Cet anticorps anti-GNAS est un anticorps Lapin Polyclonal détectant GNAS dans WB, IHC et IHC (p). Adapté pour Humain, Souris, Rat, Chien, Boeuf (Vache), Porc, Cheval, Cobaye, Singe, Roussette (Chauve-souris) et Hamster.

Aperçu rapide pour GNAS anticorps (AA 36-85) (ABIN490056)

Antigène: GNAS (GNAS Complex Locus (GNAS))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc, Cheval, Cobaye, Singe, Roussette (Chauve-souris), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GNAS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GNAS anticorps

Épitope: AA 36-85

Marque: IHC-plus™

Specificité: Human GNAS

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa36-85 of human GNAS (P63092, NP_000507). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur GNAS

Antigène: GNAS (GNAS Complex Locus (GNAS))

Autre désignation: GNAS

Sujet: Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI

ID gène: 2778

NCBI Accession: NP_000507

UniProt: Q5JWF2

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis