Tyrosinase-Related Protein 1 anticorps (AA 396-445)

N° du produit ABIN490060

Cet anticorps Lapin Polyclonal détecte spécifiquement Tyrosinase-Related Protein 1 dans IHC, WB et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Chien, Chévre, Cheval, Lapin, Mouton, Cobaye, Poisson zèbre (Danio rerio), Singe, Poulet et Roussette (Chauve-souris).

Aperçu rapide pour Tyrosinase-Related Protein 1 anticorps (AA 396-445) (ABIN490060)

Antigène: Tyrosinase-Related Protein 1 (TYRP1)

Reactivité: Humain, Souris, Rat, Chien, Chévre, Cheval, Lapin, Mouton, Cobaye, Poisson zèbre (Danio rerio), Singe, Poulet, Roussette (Chauve-souris)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tyrosinase-Related Protein 1 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Tyrosinase-Related Protein 1 anticorps

Épitope: AA 396-445

Marque: IHC-plus™

Specificité: Human TYRP1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Goat, Rabbit, Horse, Guinea pig (100%) Bovine, Pig, Chicken (92%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa396-445 of human TYRP1 (P17643, NP_000541). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Panda, Dog, Cat, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus (100%), Elephant, Bovine, Pig, Opossum, Zebra finch, Lizard (92%), Xenopus (85%), Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur Tyrosinase-Related Protein 1

Antigène: Tyrosinase-Related Protein 1 (TYRP1)

Autre désignation: TYRP1 / gp75

Sujet: Name/Gene ID: TYRP1

Synonyms: TYRP1, B-PROTEIN, CAS2, CATB, Glycoprotein 75, Hstyrrp, gp75, OCA3, SHEP11, TRP-1, Tyrosinase-related protein 1, TYRP, TYRRP, Catalase B, DHICA oxidase, Melanoma antigen gp75

ID gène: 7306

NCBI Accession: NP_000541

UniProt: P17643