LIMD1 anticorps (N-Term)

N° du produit ABIN6744453

Cet anticorps Lapin Polyclonal détecte spécifiquement LIMD1 dans WB, IHC et IHC (p). Il présente une réactivité envers Souris, Humain, Boeuf (Vache), Cobaye, Rat, Porc, Lapin, Poisson zèbre (Danio rerio), Singe et Hamster.

Aperçu rapide pour LIMD1 anticorps (N-Term) (ABIN6744453)

Antigène: LIMD1 (LIM Domains Containing 1 (LIMD1))

Reactivité: Souris, Humain, Boeuf (Vache), Cobaye, Rat, Porc, Lapin, Poisson zèbre (Danio rerio), Singe, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIMD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LIMD1 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human LIMD1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Dog (92%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human LIMD1 (Q9UGP4). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Bovine, Pig, Guinea pig, Platypus, Zebrafish (100%), Elephant, Panda, Dog, Bat, Opossum (92%), Rabbit, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur LIMD1

Antigène: LIMD1 (LIM Domains Containing 1 (LIMD1))

Autre désignation: LIMD1

Sujet: Name/Gene ID: LIMD1

Synonyms: LIMD1, LIM domains containing 1

ID gène: 8994

UniProt: Q9UGP4

Pathways: Ribonucleoprotein Complex Subunit Organization