FUBP3 anticorps (AA 503-552)

N° du produit ABIN6744454

L’anticorps Lapin Polyclonal anti-FUBP3 a été validé pour WB, IHC et IHC (p). Il convient pour détecter FUBP3 dans des échantillons de Humain, Souris, Rat, Cobaye et Chien.

Aperçu rapide pour FUBP3 anticorps (AA 503-552) (ABIN6744454)

Antigène: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Reactivité: Humain, Souris, Rat, Cobaye, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FUBP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-FUBP3 anticorps

Épitope: AA 503-552

Marque: IHC-plus™

Specificité: Human FUBP3 / FBP3

Homologie: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse, Rat, Dog (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa503-552 of human FUBP3 (Q96I24, NP_003925). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Dog (85%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur FUBP3

Antigène: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Autre désignation: FUBP3

Sujet: Name/Gene ID: FUBP3

Synonyms: FUBP3, FBP3, FUSE-binding protein 3

ID gène: 8939

NCBI Accession: NP_003925

UniProt: Q96I24