GABARAPL1 anticorps (N-Term)

N° du produit ABIN6744456

L’anticorps Lapin Polyclonal anti-GABARAPL1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter GABARAPL1 dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Porc, Poulet, Hamster, Singe et Xenopus laevis.

Aperçu rapide pour GABARAPL1 anticorps (N-Term) (ABIN6744456)

Antigène: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Porc, Poulet, Hamster, Singe, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABARAPL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GABARAPL1 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Human GABARAPL1

Homologie: Percent identity by BLAST analysis: Human, Dog, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from N-Terminus of human GABARAPL1 (Q9H0R8, NP_113600). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur GABARAPL1

Antigène: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Autre désignation: GABARAPL1 / ATG8

Sujet: Name/Gene ID: GABARAPL1

Synonyms: GABARAPL1, ATG8, ATG8L, APG8L, GABARAPL1-a, GEC-1, GEC1, APG8-LIKE, ATG8B

ID gène: 23710

NCBI Accession: NP_113600

UniProt: Q9H0R8

Pathways: Autophagy